SC6: Flocculation, Precipitation and Crystallization for Downstream Processing of Biopharmaceuticals

Wednesday 20 March, 18:30 – 21:00

Alois Jungbauer, PhD., Professor, Department of Biotechnology, University of Natural Resources Life Science, Vienna, Austria and Austrian Centre of Industrial Biotechnology
Flocculation, precipitation and crystallization alternative methods to chromatography and membrane filtration for downstream processing of biopharmaceuticals. With the advent of integrated and/or continuous processing and feed stocks with much higher titers as in the past precipitation, flocculation, and crystallization again became very attractive. These techniques are and will integrated into existing processes to intensify them. These methods are progressively integrated to new ones to establish simpler processes. In this workshop we will address the principles and methods for developing methods, scale up and large scale operation.

Following topics will be discussed in detail:

  • Overview of flocculation, precipitation and crystallization
  • Protein solubility, biophysical explanation, and measurement
  • Fundamentals of flocculation, precipitation and crystallization
  • Phase diagrams, kinetics and principles to use it for process development
  • Selection criteria for the most suited precipitant
  • Process integration, continuous operation Scale up
  • Selected examples from antibody and non-antibody world


Alois Jungbauer Alois Jungbauer is professor at the Department of Biotechnology. He teaches Protein Technology and Downstream Processing and Bioprocess Engineering. He also acts as area head and Dep. Director of Research in the Austrian Centre of Industrial Biotechnology. He is currently working in the field of bioengineering of proteins, plasmids and viruses with special focus on expression, downstream processing and characterization of large biomolecules.

He has more than 300 publications on recombinant protein production and bioseparation, 15 patents and 12 book contributions and a monograph entitled “Protein Chromatography , Process Development and Scale Up”. He is executive editor and co-founder of Biotechnology Journal, and member of editorial boards from numerous journals in the area of biochemical engineering.